Flag-tag mouse monoclonal antibody
WebHA-tag Rabbit Monoclonal Antibody staining HA tag in 293 cells transfected with C-terminal HA tagged gene by IF/ICC . Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 10% goat serum for half an hour at room temperature. ... HA Tag Mouse Monoclonal Antibody. AG8050. 50μl. Flag Tag Rabbit … WebHost Species Mouse; Clonality Monoclonal; Purification Affinity purification using immunogen. Applications WB,IF IP; Species Reactivity Hu Ms Rt; Specificity The Flag …
Flag-tag mouse monoclonal antibody
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WebThe Monoclonal ANTI-FLAG M2-Peroxidase is a mouse IgG antibody covalently conjugated to horseradish peroxidase ( HRP). The antibody binds to FLAG fusion proteins and recognizes the FLAG epitope at N-terminal, Met-N-terminal, C-terminal, and internal FLAG peptides. Packaging 0.2, 1, 1 mg Application WebThe ANTI-FLAG M2 mouse, affinity purified monoclonal antibody binds to fusion proteins containing a FLAG peptide sequence.The antibody …
WebThe FLAG™ Tag (peptide sequence DYKDDDK) is a short, ... The membrane was probed with DYKDDDDK Tag Monoclonal Antibody (L5), Alexa Fluor 488 (Cat. No. MA1-142-A488) at a dilution of 1:500 for 1 hour at room temperature on a rocking platform and washed in TBS-0.1% Tween-20. Detection was performed using a fluorescence imaging … WebA FLAG tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG tag to this protein allows one to follow the protein with an antibody against the FLAG sequence.
WebThe DYKDDDDK tag, commonly referred to as Sigma®'s FLAG® Tag, is often used as a protein modification in order to simplify the labeling and detection of proteins.This unique amino acid sequence allows for specific antibody detection in western blotting, immunoprecipitation, and immunostaining techniques.Due to the short sequence, this … WebKey features and details. PerCP Mouse monoclonal [M2] to DDDDK tag (Binds to FLAG® tag sequence) Suitable for: Flow Cyt. Reacts with: Species independent. Conjugation: PerCP. Ex: 482nm, Em: 675nm. Isotype: IgG1.
WebMonoclonal ANTI-FLAG®M1 antibody produced in mouse is suitable for immunoprecipitation, western blotting, or EIA in yeast, animal and E.colicells. Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Web> 无血清快速细胞冻存液. QuickFreezing-M. QuickFreezing-2*M greener pastures chordsWebSpecificity: Flag-Tag Mouse Monoclonal antibody detects endogenous levels of C-terminal, internal, and N-terminal Flag-tagged proteins. Immunogen: A synthesized … greener pasture in tagalogWebMonoclonal Antibody for studying Flag-Tag. Cited in 396 publications. Validated for WB, IP, IHC, IF, F. Available in 2 sizes. Highly specific and rigorously validated in-house, DYKDDDDK Tag (9A3) Mouse … greener pastures chat siteWebOct 4, 2024 · The FLAG tags are attached to the N-terminus or C-terminus of the target proteins. The tagged protein binds to the specific monoclonal antibodies conjugated on the matrix of magnetic beads or agarose gel. The residual impurities are washed off using the reagent washing buffer. flug nach newquay cornwallWebKey features and details PE Mouse monoclonal [M2] to DDDDK tag (Binds to FLAG® tag sequence) Suitable for: Flow Cyt, Sandwich ELISA Reacts with: Species independent Conjugation: PE. Ex: 488nm, Em: 575nm Isotype: IgG1 Get better batch-to-batch reproducibility with a recombinant antibody flug nach namibia ab frankfurtWeb> 无血清快速细胞冻存液. QuickFreezing-M. QuickFreezing-2*M greener pastures chicken farmWebA FLAG tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. flug nach newcastle england