NettetAfter the incubation, analyze the stained cells by flow cytometry immediately, using UV/488 nm dual excitation and measuring the fluorescence emission at 460 nm emission of Hoechst33342 and 617 nm emission of PI. Note: 1. The Hoechst 33342/PI are suspected carcinogens, operation with gloves, protective clothing, and eyewear. 2. Nettet14. apr. 2024 · F. Aplikasi diagnostik metode flow cytometry. Selain itu, pengembangan instrumen yang lebih sederhana dan terjangkau, serta peningkatan jumlah antibodi …

Antiproliferative activity and apoptosis-inducing mechanism of ...

NettetFor simultaneous flow cytometric analysis of the cell cycle, apoptotic cell death, and GFP expression, Topaz (yellow)-GFP, annexin V-PE, and PI were excited at 488 nm and Hoechst 33342 was excited at 351–356 nm. Hoechst 33342 fluorescence was collected at 460 nm using a 475 nm dichroic long pass filter and a 460/20 nm band pass filter. NettetHoechst 33342 Ready Flow Reagent is a cell-permeable, blue fluorescent DNA stain for cell cycle analysis. Add 2 drops from the dropper bottle directly into 1 x 10 6 cells, … timothy pope story

Hoechst 33342 - an overview ScienceDirect Topics

Nettet7. Analyse by flow cytometry collecting 25,000 events per sample. Live Cell Cycle Analysis with Hoechst 33342 1. Harvest cells washing in PBS. 2. Add 10 µg/ml Hoechst 33342 to cells incubate at 37oC for 45 minutes 3. Transfer cells to Falcon 5 ml tubes 4. Add 5 µg/ml PI for viability 5. Analyse by flow cytometry collecting 25,000 events per ... NettetIn flow cytometric analysis, the TO-PRO-3 (642/661) complex with nucleic acids has been excited directly by the red He-Ne laser and indirectly by the argon-ion laser by using fluorescence resonance energy transfer (FRET) from co-bound propidium iodide ( Fluorescence Resonance Energy Transfer (FRET)—Note 1.2 ). http://www.icms.qmul.ac.uk/flowcytometry/uses/cellcycleanalysis/cellcycle/index.html parted unit mib